Cell Based Assays
Apexbio Technology LLC Annexin V-Biotin/PI Apoptosis Kit 200 assays
Annexin V is a Ca2 dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS resides on the inner surface of the cell membrane s phospholipid bilayer During early stages of apoptosis it translocates from the inner leaflet to the outer surface thus exposing itself to the extracellular environment Biotin labeled Annexin V can be detected through binding with fluorescently labeled streptavidin (such as fluorescein-conjugated streptavidin or PE-conjugated streptavidin) The occurrence of apoptosis can then be identified using flow cytometry or fluorescence microscopy within only 10 to 20 minutes PI (propidium iodide) binds specifically to double-stranded DNA producing a red fluorescence signal PI cannot penetrate the intact cell membrane but late apoptotic and dead cells staining their nuclei Simultaneous staining with Annexin V-Biotin and PI permits differentiation among cells at various stages of apoptosis and dead cells
Revvity Health Sciences Inc HTRF Phospho and Total protein blocking reagent, 2 ml
HTRF Phospho and Total protein blocking reagent, 2 ml
Abcam AGE (Advanced Glycation End) Assay Kit
AGE (Advanced Glycation End) Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
STEMCELL Technologies Resazurin (Sodium Salt), Size: 5 g
Resazurin (7-hydroxy-10-oxidophenoxazin-10-ium-3-one, sodium) is a blue fluorogenic dye used as a redox indicator in cell viability and proliferation assays for bacteria, yeast or mammalian cells. The blue form of the dye is irreversibly reduced by enzymes in viable cells to generate a highly red-fluorescent product, Resazurin, which exhibits an emission maximum at ~580 nm and can be detected by flow cytometry, fluorescence microscopy, and high-throughput screening methods. Resazurin is minimally toxic to living cells, making it suitable for use in long-term cell culture. The dye has also been used to assay L-glutamate and to measure the metabolic activity of mitochondria.
Meso Scale Discovery Canine ProInflammatory Panel 3 Ultra-Sensitive Kit
This item has a minimum order quantity of 5 packs per supplier requirements. Cytokines & Chemokines , Immunology/Inflammationm, Canine, 96 wells
Agilent Technologies Seahorse XF Glycolytic Rate Assay Kit
Seahorse XF Glycolytic Rate Assay Kit. Each kit contains six (6) single-use pouches. Each pouch contains one (1) each of rotenone/antimycin A and 2-deoxy-D-glucose. For use with XFe/XF96 and XFe24 analyzers. Not compatible with XF24 and XF24-3 analyzers.
Cellular Dynamics International iCell Motor Neurons Kit 01279, Human iPSC-derived spinal motor neurons, ≥90% β-III tubulin+, ≥70% ISL1/2+, Viability ≥14 days, 3 x 10^6 cells/vial
iCell Motor Neurons Kit 01279 is a highly pure, functional population of human spinal motor neurons derived from induced pluripotent stem cells (iPSCs) by FUJIFILM Cellular Dynamics Inc. This kit offers a reliable source of human motor neurons with typical morphology, strong viability in culture, and consistent gene expression. These cells are an essential resource for scientific applications, including neurodegenerative disease research, drug development, and toxicology screening.
- Human iPSC-derived spinal motor neurons
- Purity ≥90% β-III tubulin+, ≥70% ISL1/2+ at Day 14
- Viability in culture for ≥14 days
- Typical motor neuron morphology with branching neurites
- Key markers expressed: SMI32
- Applications include neurodegenerative disease research, drug discovery, and bioengineering
- Quantity: 3 x 10^6 cells/vial
Abcam Neuraminidase Assay Kit (Fluorometric - Blue)
Neuraminidase Assay Kit (Fluorometric - Blue)
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Heme Assay Kit sufficient
Heme is a member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm and is a key prosthetic group for various essential proteins such as hemoglobin cytochromes catalases and peroxidases. free heme which can be released from hemoglobin following hemolysis is pro-inflammatory and contributes to iron-derived reactive oxygen species. Heme determination is widely practiced by researchers of various blood diseases.
Sigma Aldrich Fine Chemicals Biosciences Bile Acid Assay Kit suffic
Twelve different types of bile acids are typically found in mammals among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. finally these four can be conjugated to either taurine or glycine creating 8 different conjugated bile acids. Bile acid levels in feces blood urine and bile can be used as markers for various diseases such as hyperlipidemia cholestasis gall stones colon cancer etc. Bile acids also exist as sulfate salt forms known as bile acid sulfates. Sulfation of bile acids increases their solubility and decreases intestinal absorption thereby enhancing fecal and urinary excretion.